Immediate increase of plasma protein complement C3 in response to an acute stressor.

Whereas chronic stress has immunosuppressive effects, the more immediate immunologic consequences of acute stressors are less known. We postulated that, as part of their 'fight or flight' response, rainbow trout would rapidly increase the efficacy of their natural immune system by means of increased concentrations of crucial plasma proteins. Plasma samples were taken from resting fish and from fish 5, 10 or 20 min after initiation of a stressful regime. Using crossed immunoelectrophoresis, we documented increases in concentrations of complement C3 and 3 other proteins within 5 min of initiation of stress. The concentration of C3 nearly doubled within 10 min of initiation of stress and had returned to near resting level by 20 min. This rapid kinetics preclude dependence on gene activation, the basis of the acute phase response. Potentiation of natural immunity, which can reasonably be expected to be selectively advantageous during or immediately after acute stressors may be one result of this increase.

Effects of extracellular products of Vibrio vulnificus on Acanthopagrus schlegeli serum components in vitro and in vivo.

A Vibrio strain Ls001, originally isolated from a body surface lesion of a moribund black porgy (Acanthopagrus schlegeli) in 1994 in Taiwan, was identified as Vibrio vulnificus. The extracellular products (ECP) of the strain were lethal to the fish, and its effects on fish serum in vitro and in vivo are described in the present study. Nine major precipitation arcs were visualized in normal fish serum in a crossed immunoelectrophoresis (CIE) gel using rabbit antiserum to the fish normal serum and staining with Coomassie brilliant blue. Only four and six of the nine major arcs could be tentatively identified by CIE following in vivo and in vitro ECP treatment, respectively. The same two major arcs were both missing following either in vivo or in vitro treatment with ECP. These complex events may significantly contribute to the pathogenesis of V. vulnificus in A. schlegeli.

Immunohistochemical identification of aetiological agents of systemic bovine zygomycosis.

The reactivity of a panel of rabbit hyperimmune antisera raised against somatic antigens of three zygomycetes, Absidia corymbifera, Rhizopus oryzae and Rhizomucor (Mucor) pusillus was assessed by crossed immunoelectrophoresis (XIE) and indirect immunofluorescence (IIF) staining of experimentally infected murine tissues. Despite a widespread cross-reactivity of the neat antisera, heterologous absorption rendered antisera monospecific as assessed by XIE. Heterologous absorption also rendered the antisera against R. oryzae and R. (Mucor) pusillus specific by IIF, whereas heterologous absorption of Abs. corymbifera antiserum did not abolish reactivity with R. oryzae. The reactivity of the heterologously absorbed antisera and a murine monoclonal IgG1 antibody against Abs. corymbifera enabled zygomycetes within bovine lesions to be identified. Of 113 zygomycotic lesions of 95 cattle examined, 82 (72.5 per cent) showed a pattern of reactivity compatible with Abs. corymbifera, 21 (18.6 per cent) with R. oryzae, and 5 (4.4 per cent) with R. (Mucor) pusillus. Fungi in four lesions reacted with the monoclonal antibody only, and fungal elements in one lesion showed no reactivity at all. Each of the bovine lesions contained only a single fungal species, but one animal was infected by Abs. corymbifera in the rumen and by R. oryzae in the other stomach compartments. Apart from being the main cause of systemic bovine zygomycosis in Denmark, Abs. corymbifera also seems to be the most pathogenic due to its frequent haematogenous and lymphatic spread (87.8 per cent) as compared with R. oryzae (1.4 per cent) and R. (Mucor) pusillus (5.4 per cent).

Identification of antigens for use in immunodiagnosis of Taenia saginata cysticercosis.

Fifteen metacestode antigens from Taenia saginata were defined by Laurell crossed immunoelectrophoresis and investigated for their potential use in immunodiagnosis of bovine cysticercosis. Several antigens cross reacted with those of some common cattle parasites. Three of the antigens, designated as numbers 4, 8 and 11, were selected on the basis of their restricted cross reactions and were isolated by affinity chromatography. These antigens showed high sensitivity and specificity values in the enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of bovine cysticercosis.